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Biological Sciences: genetics > molecular genetics > genetic techniques and protocols
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17631 items, grouped by Biological Sciences (view ungrouped items)

chromosome banding (26)   
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Identification of the unculturable bacteria Candidatus arthromitus in the intestinal content of trouts using Dot blot and Southern blot techniques
Veterinary microbiology

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Immunodetection of coproantigens for the diagnosis of amphistomosis in naturally infected Indian Water Buffalo, Bubalus bubalis
Veterinary parasitology

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Karyotype analysis of Oreochromis mossambicus, O. urolepis hornorum and their hybrid based on Cot-1 DNA bands by fluorescence in situ hybridization
Aquaculture research

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Chromosomes analysis of five diploid garden Hyacinth species
Scientia horticulturae

all 26 items...

chromosome mapping (1542)   
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A genetic linkage map for tef [Eragrostis tef (Zucc.) Trotter]
Theoretical and applied genetics

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A QTL study on late leaf spot and rust revealed one major QTL for molecular breeding for rust resistance in groundnut (Arachis hypogaea L.)
Theoretical and applied genetics

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Advances in Mapping Loci Conferring Resistance to Rice Sheath Blight and Mining Rhizoctonia solani Resistant Resources
Rice science

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Epigenetic chromatin modifiers in barley: I. Cloning, mapping and expression analysis of the plant specific HD2 family of histone deacetylases from barley, during seed development and after hormonal treatment
Physiologia plantarum

all 1542 items...

cytogenetic analysis (197)   
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78,XX Testicular DSD Syndrome in a Mongrel Dog
Reproduction in domestic animals

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Chiral mutagens: Cytogenetic effects on higher plants
Cytology and Genetics

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Characterisation of commercial and natural Torulaspora delbrueckii wine yeast strains
International journal of food microbiology

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Clinical genealogical and molecular genetic study of patients with mental retardation
Cytology and Genetics

all 197 items...

diallel analysis (38)   
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Maternal and paternal effects on the germination time of non-dormant seeds of a monocarpic perennial species, Aster kantoensis (Compositae)
Plant species biology

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Low S-allele numbers limit mate availability, reduce seed set and skew fitness in small populations of a self-incompatible plant
Journal of applied ecology

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Genetic analysis for resistance to bean fly (Ophiomyia phaseoli) and seed yield among common bean genotypes in a semi-arid environment
Field crops research

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Overview of QTL detection in plants and tests for synergistic epistatic interactions
Genetica

all 38 items...

direct DNA uptake (4)   
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Delivery of dsRNA for RNAi in insects: an overview and future directions
Insect science

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Transformation of the limonene synthase gene into peppermint (Mentha piperita L.) and preliminary studies on the essential oil profiles of single transgenic plants
Theoretical and applied genetics

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Improvement of transformation efficiency by bioactive-beads-mediated gene transfer using DNA-lipofectin complex as entrapped genetic material
Journal of bioscience and bioengineering

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Transient gene expression in guard cell chloroplasts of tobacco using ArF excimer laser microablation
Journal of bioscience and bioengineering

DNA footprinting (1)   
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The heat-shock element is a functional component of the Arabidopsis APX1 gene promoter
Plant physiology

electroporation (55)   
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Microscopic visualization of Pulsed Electric Field induced changes on plant cellular level
Innovative food science and emerging technologies

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Development of a Genetic System for the Chemolithoautotrophic Bacterium Thiobacillus denitrificans
Applied and environmental microbiology AEM

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Effect of apparent density of sliced food particles on the efficiency of pulsed electric field treatment
Innovative food science and emerging technologies

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Osteological and histopathological details of unilateral microphthalmia and anophthalmia in juvenile common carp (Cyprinus carpio)
Aquaculture

all 55 items...

gene targeting (432)   
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Contrasting Community versus Population-Based Estimates of Grazing and Virus-Induced Mortality of Phytoplankton
Microbial ecology

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Outcome of Colonization of Apis mellifera by Nosema ceranae
Applied and environmental microbiology AEM

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Molecular frequency and isolation of cyst-forming coccidia from free ranging chickens in Bahia State, Brazil
Veterinary parasitology

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A single transgene locus triggers both transcriptional and post-transcriptional silencing through double-stranded RNA production
Planta

all 432 items...

gene transfer (879)   
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High transfection efficiency of porcine peripheral blood T cells via nucleofection
Veterinary immunology and immunopathology

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Transcriptional activation of the maize endosperm transfer cell-specific gene BETL1 by ZmMRP-1 is enhanced by two C₂H₂ zinc finger-containing proteins
Planta

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Rapid and Cost-Effective Gene Targeting in Rat Embryonic Stem Cells by TALENs
Journal of genetics and genomics

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Single dose adenovirus vectored vaccine induces a potent and long-lasting immune response against rabbit hemorrhagic disease virus after parenteral or mucosal administration
Veterinary immunology and immunopathology

all 879 items...

genetic complementation test (15)   
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Molecular characterization of a nuclear topoisomerase II from Nicotiana tabacum that functionally complements a temperature-sensitive topoisomerase II yeast mutant
Plant molecular biology

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A plasma membrane zinc transporter from Medicago truncatula is up-regulated in roots by Zn fertilization, yet down-regulated by arbuscular mycorrhizal colonization
Plant molecular biology

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A gateway cloning vector set for high-throughput functional analysis of genes in planta
Plant physiology

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LAF3, a novel factor requried for normal phytochrome a signaling
Plant physiology

all 15 items...

genetic markers (2889)   
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Study on differential expression of 1-aminocyclopropane-1-carboxylic acid oxidase genes in table grape cv. Thompson Seedless
Postharvest biology and technology

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Comparison of anonymous and targeted molecular markers for the estimation of genetic diversity in ex situ conserved Lactuca
Theoretical and applied genetics

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Statistical power for detecting genetic divergence—organelle versus nuclear markers
Conservation genetics

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Selective genotyping to identify late blight resistance genes in an accession of the tomato wild species Solanum pimpinellifolium
Euphytica

all 2889 items...

in situ hybridization (785)   
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An oligonucleotide prokaryotic acidophile microarray: its validation and its use to monitor seasonal variations in extreme acidic environments with total environmental RNA
Environmental microbiology

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Halophilic archaea in the human intestinal mucosa
Environmental microbiology

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Linkages between bacterioplankton community composition, heterotrophic carbon cycling and environmental conditions in a highly dynamic coastal ecosystem
Environmental microbiology

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Macromolecular organization and genetic mapping of a rapidly evolving chromosome-specific tandem repeat family (B77) in cotton (Gossypium)
Plant molecular biology

all 785 items...

microarray technology (881)   
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An oligonucleotide prokaryotic acidophile microarray: its validation and its use to monitor seasonal variations in extreme acidic environments with total environmental RNA
Environmental microbiology

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Genetic and Genomic Insights into the Role of Benzoate-Catabolic Pathway Redundancy in Burkholderia xenovorans LB400
Applied and environmental microbiology AEM

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Genomic resources for the brown planthopper, Nilaparvata lugens: Transcriptome pyrosequencing and microarray design
Insect science

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Cell Type-Specific Transcriptome Analysis of the Soybean Leaf Paraveinal Mesophyll Layer
Plant molecular biology reporter

all 881 items...

molecular cloning (1140)   
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An oligonucleotide prokaryotic acidophile microarray: its validation and its use to monitor seasonal variations in extreme acidic environments with total environmental RNA
Environmental microbiology

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REP27, a Tetratricopeptide Repeat Nuclear-Encoded and Chloroplast-Localized Protein, Functions in D1/32-kD Reaction Center Protein Turnover and Photosystem II Repair from Photodamage
Plant physiology

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Lactobacillus acidophilus as a live vehicle for oral immunization against chicken anemia virus
Applied microbiology and biotechnology.

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Growth hormone gene polymorphisms in tench, Tinca tinca L.
Aquaculture

all 1140 items...

Northern blotting (207)   
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Selectable antibiotic resistance marker gene-free transgenic rice harbouring the garlic leaf lectin gene exhibits resistance to sap-sucking planthoppers
Plant cell reports

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Expression of a lipid-inducible, self-regulating form of Yarrowia lipolytica lipase LIP2 in Saccharomyces cerevisiae
Applied microbiology and biotechnology.

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Effects of water-deficit stress on the transcriptomes of developing immature ear and tassel in maize
Plant cell reports

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A long antisense RNA in plant chloroplasts
The new phytologist

all 207 items...

nucleic acid hybridization (264)   
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Verminephrobacter aporrectodeae sp. nov. subsp. tuberculatae and subsp. caliginosae, the specific nephridial symbionts of the earthworms Aporrectodea tuberculata and A. caliginosa
Antonie van Leeuwenhoek

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Highly efficient sorghum transformation
Plant cell reports

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Devosia epidermidihirudinis sp. nov. isolated from the surface of a medical leech
Antonie van Leeuwenhoek

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Updating the taxonomic toolbox: classification of Alteromonas spp. using multilocus phylogenetic analysis and MALDI-TOF mass spectrometry
Antonie van Leeuwenhoek

all 264 items...

plasmid curing (9)   
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Extrachromosomal, extraordinary and essential—the plasmids of the Roseobacter clade
Applied microbiology and biotechnology

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Assessment and characterization of heavy metal resistance in Palk Bay sediment bacteria
Marine environmental research

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Flavonoid‐induced calcium signalling in Rhizobium leguminosarum bv. viciae
The new phytologist

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Detection of florfenicol resistance genes in Riemerella anatipestifer isolated from ducks and geese
Veterinary microbiology

all 9 items...

polymerase chain reaction (8368)   
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Anaplasma phagocytophilum infection in a domestic cat in Finland: Case report
Acta veterinaria scandinavica

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Effect of Mn²⁺ augmentation on reinforcing aerobic sludge granulation in a sequencing batch reactor
Applied microbiology and biotechnology

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Contrasting Community versus Population-Based Estimates of Grazing and Virus-Induced Mortality of Phytoplankton
Microbial ecology

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Unexpected Stability of Bacteroidetes and Firmicutes Communities in Laboratory Biogas Reactors Fed with Different Defined Substrates
Applied and environmental microbiology AEM.

all 8368 items...

sequence alignment (321)   
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Isolation and molecular characterization of a second serotype of the encephalomyocarditis virus
Veterinary microbiology

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Changes in cellular macromolecules (DNA, RNA and protein) during seed germination in tomato, following the use of a butenolide, isolated from plant-derived smoke
Plant growth regulation

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Molecular Identification of a ‘Candidatus Phytoplasma ziziphi'-related Strain Infecting Amaranth (Amaranthus retroflexus L.) in China
Phytopathologische Zeitschrift

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Genetic characterization and phylogenetic analysis of Trypanosoma evansi in Iranian dromedary camels
Parasitology research

all 321 items...

sequence analysis (2794)   
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16S-23S rRNA Intergenic Spacer Region Variability in the Genus Frankia
Microbial ecology

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A new satellite RNA is associated with natural infections of cucumber mosaic virus in succulent snap bean
Archives of virology.

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Identification of Poly(cis-1,4-Isoprene) Degradation Intermediates during Growth of Moderately Thermophilic Actinomycetes on Rubber and Cloning of a Functional lcp Homologue from Nocardia farcinica Strain E1
Applied and environmental microbiology AEM

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Uncovering of major genetic factors generating naturally occurring variation in heading date among Asian rice cultivars
Theoretical and applied genetics

all 2794 items...

sexing (116)   
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Use of a modified form factor to compare condition among North American lake sturgeon stocks
Zeitschrift fur angewandte Ichthyologie

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Effects of the estrogen mimic genistein as a dietary component on sex differentiation and ethoxyresorufin-O-deethylase (EROD) activity in channel catfish (Ictalurus punctatus)
Fish physiology and biochemistry

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Reduction of Oxidative Stress in Bovine Spermatozoa During Flow Cytometric Sorting
Reproduction in domestic animals

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Electrophysiological and Behavioral Responses of the Bark Beetle Dendroctonus rhizophagus to Volatiles from Host Pines and Conspecifics
Journal of chemical ecology

all 116 items...

Southern blotting (305)   
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A simplified protocol for preparing DNA from filamentous cyanobacteria
Plant molecular biology reporter

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Highly efficient sorghum transformation
Plant cell reports

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A papaya-specific gene, papain, used as an endogenous reference gene in qualitative and real-time quantitative PCR detection of transgenic papayas
European food research and technology

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An efficient method for the production of marker-free transgenic plants of peanut (Arachis hypogaea L.)
Plant cell reports

all 305 items...

 (others) (642)   
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Genetic analysis of seed mineral accumulation affected by phosphorus deprivation in Brassica napus
Euphytica

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Evidence for isolation by time in the European eel (Anguilla anguilla L.)
Molecular ecology

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Population genetic structure and long‐distance dispersal among seabird populations: Implications for colony persistence
Molecular ecology

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Molecular characterization of Pseudomonas syringae isolates from fruit trees and raspberry in Serbia
European journal of plant pathology

all 642 items...