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Biological Sciences: genetics > molecular genetics > genetic engineering > protein engineering
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204 items, grouped by Calendar Year (view ungrouped items)

Year (204)   
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Identifying Functionally Important Mutations from Phenotypically Diverse Sequence Data
Applied and environmental microbiology AEM

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Identification of a C2H 2-type zinc finger transcription factor (ZAT10) from Arabidopsis as a substrate of MAP kinase
Plant cell reports

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Combination of site-directed mutagenesis and calcium ion addition for enhanced production of thermostable MBP-fused heparinase I in recombinant Escherichia coli
Applied microbiology and biotechnology

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Characterization of (R)-2-Hydroxyisocaproate Dehydrogenase and a Family III Coenzyme A Transferase Involved in Reduction of L-Leucine to Isocaproate by Clostridium difficile
Applied and environmental microbiology AEM

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Evolution of Cinnamate/p-Coumarate Carboxyl Methyltransferases and Their Role in the Biosynthesis of Methylcinnamate
The plant cell

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Molecular Determinants of the Cofactor Specificity of Ribitol Dehydrogenase, a Short-Chain Dehydrogenase/Reductase
Applied and environmental microbiology AEM.

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Fungal aryl-alcohol oxidase: a peroxide-producing flavoenzyme involved in lignin degradation
Applied microbiology and biotechnology

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Role of Tyr-435 of Vibrio harveyi Chitinase A in Chitin Utilization
Applied biochemistry and biotechnology

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Alteration of Leucine Aminopeptidase from Streptomyces septatus TH-2 to Phenylalanine Aminopeptidase by Site-Directed Mutagenesis
Applied and environmental microbiology AEM

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Proteomic understanding of intracellular responses of recombinant Chinese hamster ovary cells cultivated in serum-free medium supplemented with hydrolysates
Applied microbiology and biotechnology.

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Isolation and functional characterization of an influx silicon transporter in two pumpkin cultivars contrasting in silicon accumulation
The plant journal

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Arginine Residues in the C-terminal and their Relationship with the Analgesic Activity of the Toxin from the Chinese Scorpion Buthus martensii Karsch (BmK AGP-SYPU1)
Applied biochemistry and biotechnology

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A rapid assay to quantify the cleavage efficiency of custom-designed nucleases in planta
Plant molecular biology

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Fungal chitinases: diversity, mechanistic properties and biotechnological potential
Applied microbiology and biotechnology.

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Xylanase XYL1p from Scytalidium acidophilum: Site-directed mutagenesis and acidophilic adaptation
Bioresource technology

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Characterization of Xyn10J, a Novel Family 10 Xylanase from a Compost Metagenomic Library
Applied biochemistry and biotechnology

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Fumarate and cytosolic pH as modulators of the synthesis or consumption of C₄ organic acids through NADP-malic enzyme in Arabidopsis thaliana
Plant molecular biology

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Compensation of the enantioselectivity-activity trade-off in the directed evolution of an esterase from Rhodobacter sphaeroides by site-directed saturation mutagenesis
Applied microbiology and biotechnology

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Analysis of a Ferric Uptake Regulator (Fur) Mutant of Desulfovibrio vulgaris Hildenborough
Applied and environmental microbiology AEM

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Biocatalytic Conversion of Avermectin to 4''-Oxo-Avermectin: Improvement of Cytochrome P450 Monooxygenase Specificity by Directed Evolution
Applied and environmental microbiology AEM

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Introduction of sulfhydryl groups and disulfide linkage to mungbean 8Sα globulin and effects on physicochemical and functional properties
Food research international

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Biochemical and genetic analysis of a cutinase-type polyesterase from a thermophilic Thermobifida alba AHK119
Applied microbiology and biotechnology.

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Cloning and characterization of a novel amidase from Paracoccus sp. M-1, showing aryl acylamidase and acyl transferase activities
Applied microbiology and biotechnology.

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Engineering lower inhibitor affinities in β-D-xylosidase of Selenomonas ruminantium by site-directed mutagenesis of Trp145
Journal of industrial microbiology and biotechnology

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Kinetic analysis of maize glutathione S-transferase I catalysing the detoxification from chloroacetanilide herbicides
Planta

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Affinity of Avr2 for tomato cysteine protease Rcr3 correlates with the Avr2-triggered Cf-2-mediated hypersensitive response
Molecular plant pathology

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Tuning the substrate selectivity of meta-cleavage product hydrolase by domain swapping
Applied microbiology and biotechnology

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Features and technical applications of ω-transaminases
Applied microbiology and biotechnology.

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Functional characterization of Class II 5-enopyruvylshikimate-3-phosphate synthase from Halothermothrix orenii H168 in Escherichia coli and transgenic Arabidopsis
Applied microbiology and biotechnology.

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Involvement of the cylindrical inclusion (CI) protein in the overcoming of an eIF4E-mediated resistance against Lettuce mosaic potyvirus
Molecular plant pathology

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Crystal structure of Clostridium thermocellum ribose-5-phosphate isomerase B reveals properties critical for fast enzyme kinetics
Applied microbiology and biotechnology.

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Two O-methyltransferases from Picea abies: characterization and molecular basis of different reactivity
Planta

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The interconversion of ACC deaminase and d-cysteine desulfhydrase by directed mutagenesis
Planta

(Click to View)
Protein engineering and discovery of lipases
European journal of lipid science and technology

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Molecular Modeling and Site-Directed Mutagenesis Reveal the Benzylisoquinoline Binding Site of the Short-Chain Dehydrogenase/Reductase Salutaridine Reductase
Plant physiology

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Changes in the Catalytic Properties of Pyrococcus furiosus Thermostable Amylase by Mutagenesis of the Substrate Binding Sites
Applied and environmental microbiology AEM

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Plant Glutathione Peroxidases Are Functional Peroxiredoxins Distributed in Several Subcellular Compartments and Regulated during Biotic and Abiotic Stresses
Plant physiology

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Identification of Features Regulating OST1 Kinase Activity and OST1 Function in Guard Cells
Plant physiology

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Fusion of a Novel Genetically Engineered Chitosan Affinity Protein and Green Fluorescent Protein for Specific Detection of Chitosan In Vitro and In Situ
Applied and environmental microbiology AEM.

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How is the reactivity of laccase affected by single-point mutations? Engineering laccase for improved activity towards sterically demanding substrates
Applied microbiology and biotechnology.

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Residue mutations in the sweetness loops for the sweet-tasting protein brazzein
Food chemistry

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Metabolically engineered soybean seed with enhanced threonine levels: biochemical characterization and seed-specific expression of lysine-insensitive variants of aspartate kinases from the enteric bacterium Xenorhabdus bovienii
Plant biotechnology journal

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Microsatellite polymorphism is likely involved in phytoene synthase activity in Citrus
Plant cell, tissue, and organ culture

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Site-directed mutagenesis of the Arabidopsis heterotrimeric G protein β subunit suggests divergent mechanisms of effector activation between plant and animal G proteins
Planta

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Simple and efficient expression of Agaricus meleagris pyranose dehydrogenase in Pichia pastoris
Applied microbiology and biotechnology.

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Purification, cloning, functional expression and characterization of perakine reductase: the first example from the AKR enzyme family, extending the alkaloidal network of the plant Rauvolfia
Plant molecular biology

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New strategy of site-directed mutagenesis identifies new sites to improve Streptomyces clavuligerus deacetoxycephalosporin C synthase activity toward penicillin G
Applied microbiology and biotechnology

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Cloning and sequencing of the α-amylase gene from bacillus subtilis us116 strain encoding an enzyme closely identical to that from bacillus amyloliquefaciens but distinct in thermal stability
Journal of food biochemistry

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Site-directed mutagenesis of a tetrameric dandelion polyphenol oxidase (PPO-6) reveals the site of subunit interaction
Plant molecular biology

(Click to View)
Characterization of the necrosis determinant of the European genotype of pepino mosaic virus by site-specific mutagenesis of an infectious cDNA clone
Archives of virology.

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A novel low-temperature-active β-glucosidase from symbiotic Serratia sp. TN49 reveals four essential positions for substrate accommodation
Applied microbiology and biotechnology.

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Molecular Architecture of Strictosidine Glucosidase: The Gateway to the Biosynthesis of the Monoterpenoid Indole Alkaloid Family
The plant cell

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Approaches to achieve high-level heterologous protein production in plants
Plant biotechnology journal

(Click to View)
The molecular biology of rotaviruses X: intercellular dissemination of rotavirus NSP4 requires glycosylation and is mediated by direct cell-cell contact through cytoplasmic extrusions
Archives of virology.

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Transformation of the Green Alga Haematococcus pluvialis with a Phytoene Desaturase for Accelerated Astaxanthin Biosynthesis
Applied and environmental microbiology AEM

(Click to View)
Genetic Modification of the Penicillin G Acylase Surface To Improve Its Reversible Immobilization on Ionic Exchangers
Applied and environmental microbiology AEM

(Click to View)
Identification of activity related amino acid mutations of a GH9 termite cellulase
Bioresource technology

(Click to View)
A single amino acid substitution in PthA of Xanthomonas axonopodis pv. citri altering canker formation on grapefruit leaves
European journal of plant pathology

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Active site analysis of cis-epoxysuccinate hydrolase from Nocardia tartaricans using homology modeling and site-directed mutagenesis
Applied microbiology and biotechnology

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Current state and perspectives on erythropoietin production
Applied microbiology and biotechnology.

(Click to View)
Mitsuaria chitosanase with unrevealed important amino acid residues: characterization and enhanced production in Pichia pastoris
Applied microbiology and biotechnology

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Simultaneous substitution of Gly96 to Ala and Ala183 to Thr in 5-enolpyruvylshikimate-3-phosphate synthase gene of E. coli (k12) and transformation of rapeseed (Brassica napus L.) in order to make tolerance to glyphosate
Plant cell reports

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Weak Activity of Haloalkane Dehalogenase LinB with 1,2,3-Trichloropropane Revealed by X-Ray Crystallography and Microcalorimetry
Applied and environmental microbiology AEM

(Click to View)
A high-throughput screening system for the evaluation of biomass-hydrolyzing glycoside hydrolases
Bioresource technology

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Extracellular metalloproteases from bacteria
Applied microbiology and biotechnology.

(Click to View)
Further Stabilization of Leu155 Mutant Thermolysins by Mutation of an Autodegradation Site
Applied biochemistry and biotechnology

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Three amino acid changes contribute markedly to the thermostability of β-glucosidase BglC from Thermobifida fusca
Bioresource technology

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Thermostable Bacterial Endoglucanases Mined from Swiss-Prot Database
Applied biochemistry and biotechnology

(Click to View)
Reduction of U(VI) by the deep subsurface bacterium, Thermus scotoductus SA-01, and the involvement of the ABC transporter protein
Chemosphere

(Click to View)
Enhancement of the thermostability of the maltogenic amylase MAUS149 by Gly312Ala and Lys436Arg substitutions
Bioresource technology

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Purification of soluble and active RaxH, a transmembrane histidine protein kinase from Xanthomonas oryzae pv. oryzae required for AvrXa21 activity
Molecular plant pathology

(Click to View)
Use of Random and Saturation Mutageneses To Improve the Properties of Thermus aquaticus Amylomaltase for Efficient Production of Cycloamyloses
Applied and environmental microbiology AEM

(Click to View)
Thiol-Based Regulation of Redox-Active Glutamate-Cysteine Ligase from Arabidopsis thaliana
The plant cell

(Click to View)
Engineering of polyhydroxyalkanoate (PHA) synthase PhaC2Ps of Pseudomonas stutzeri via site-specific mutation for efficient production of PHA copolymers
Applied microbiology and biotechnology.

(Click to View)
New Tools for Exploring “Old Friends—Microbial Lipases”
Applied biochemistry and biotechnology

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Analysis of Amino Acid Residues Involved in Catalysis of Polyethylene Glycol Dehydrogenase from Sphingopyxis terrae, Using Three-Dimensional Molecular Modeling-Based Kinetic Characterization of Mutants
Applied and environmental microbiology AEM

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Identification of Pyruvate Carboxylase Genes in Pseudomonas aeruginosa PAO1 and Development of a P. aeruginosa-Based Overexpression System for α₄- and α₄β₄-Type Pyruvate Carboxylases
Applied and environmental microbiology AEM

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Effects of engineered methionine in the 8Sα globulin of mungbean on its physicochemical and functional properties and potential nutritional quality
Food research international

(Click to View)
Multiple mutations of the critical amino acid residues for the sweetness of the sweet-tasting protein, brazzein
Food chemistry

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A Novel Lactone-Forming Carboxylesterase: Molecular Identification of a Tuliposide A-Converting Enzyme in Tulip
Plant physiology

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Altering the scissile fatty acid binding site of Candida antarctica lipase A by protein engineering for the selective hydrolysis of medium chain fatty acids
European journal of lipid science and technology

(Click to View)
Enhancement of riboflavin production with Bacillus subtilis by expression and site-directed mutagenesis of zwf and gnd gene from Corynebacterium glutamicum
Bioresource technology

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Cellulose Binding Domains of a Phytophthora Cell Wall Protein Are Novel Pathogen-Associated Molecular Patterns
The plant cell

(Click to View)
Inhibition of Cryptosporidium parvum infection of a mammalian cell culture by recombinant scFv antibodies
Antonie van Leeuwenhoek

(Click to View)
Crystal structures of rice (Oryza sativa) glyceraldehyde-3-phosphate dehydrogenase complexes with NAD and sulfate suggest involvement of Phe37 in NAD binding for catalysis
Plant molecular biology

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Engineering a novel c-di-GMP-binding protein for biofilm dispersal
Environmental microbiology

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Characterization of a family 5 glycoside hydrolase isolated from the outer membrane of cellulolytic Cytophaga hutchinsonii
Applied microbiology and biotechnology

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A targeted gene knockout method using a newly constructed temperature-sensitive plasmid mediated homologous recombination in Bifidobacterium longum
Applied microbiology and biotechnology.

(Click to View)
Biocatalytic synthesis of optically active tertiary alcohols
Applied microbiology and biotechnology.

(Click to View)
The targeting of the oxysterol-binding protein ORP3a to the endoplasmic reticulum relies on the plant VAP33 homolog PVA12
The plant journal

(Click to View)
Functional analysis of NLP genes from Botrytis elliptica
Molecular plant pathology

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Lysine Decarboxylase Catalyzes the First Step of Quinolizidine Alkaloid Biosynthesis and Coevolved with Alkaloid Production in Leguminosae
The plant cell

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Protein Engineering of the 4-Methyl-5-Nitrocatechol Monooxygenase from Burkholderia sp. Strain DNT for Enhanced Degradation of Nitroaromatics
Applied and environmental microbiology AEM

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Novel intracellular GH10 xylanase from Cohnella laeviribosi HY-21: Biocatalytic properties and alterations of substrate specificities by site-directed mutagenesis of Trp residues
Bioresource technology

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A CELLULOSE SYNTHASE (CESA) gene essential for gametophore morphogenesis in the moss Physcomitrella patens
Planta

(Click to View)
Identification and Mutational Analysis of Arabidopsis FLS2 Leucine-Rich Repeat Domain Residues That Contribute to Flagellin Perception
The plant cell

(Click to View)
Engineering of LadA for enhanced hexadecane oxidation using random- and site-directed mutagenesis
Applied microbiology and biotechnology.

(Click to View)
Molecular and catalytic characterization of a phi class glutathione transferase from Cathaya argyrophylla
Biochemical systematics and ecology

(Click to View)
Structural and functional characterization of a novel, host penetration-related pectate lyase from the potato cyst nematode Globodera rostochiensis
Molecular plant pathology

all 204 items...