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more general categories information about this item Biological Sciences Biological Sciences biochemistry (102540) biochemical compounds (82890) amino acids, peptides and proteins (29753) amino acids (5838) serine (239) carbohydrates (23127) polysaccharides (9609) glycosaminoglycans (125) heparin (50) enzymes (23169) lyases (1475) enzymology (9123) enzyme activity (7588) genetics (60992) molecular genetics (44734) genetic engineering (682) protein engineering (204) site-directed mutagenesis (166) mutation (5459) mutagenesis (625) site-directed mutagenesis (166) Breeding and Genetic Improvement Breeding and Genetic Improvement genetic engineering (682) protein engineering (204) site-directed mutagenesis (166) Calendar Year Calendar Year Year (337475) 2013 (32153) Economics, Business and Industry Economics, Business and Industry economics (27895) industrial organization and market structure (7082) industry (6905) industrial applications (305) Health and Pathology Health and Pathology animal and human health (42466) medical sciences (24631) pharmacology (12576) drugs (10189) anticoagulants (103) heparin (50) Physical and Chemical Sciences Physical and Chemical Sciences chemical substances (129118) biochemical compounds (82878) amino acids, peptides and proteins (29751) amino acids (5838) serine (239) carbohydrates (23127) polysaccharides (9609) glycosaminoglycans (125) heparin (50) enzymes (23169) lyases (1475) chemical compounds (78840) acids, bases, and salts (29068) salts (12335) inorganic salts (10494) sulfates (1482) inorganic compounds (30695) inorganic salts (10493) sulfates (1482) metals (14084) metallic elements (11806) alkaline earth metals (2686) calcium (2248) elementary particles (4007) ions (2322) elements (30672) metallic elements (11806) alkaline earth metals (2686) calcium (2248) technical chemicals (15701) stabilizers (137) chemistry (84737) physicochemical properties (27179) molecular weight (2511) thermal stability (959) physics (54990) physical properties (32618) thermal properties (1570) thermal stability (959) thermodynamics (5162) thermal properties (1570) thermal stability (959) Reference Types Reference Types Journal (337546) Research, Technology and Engineering Research, Technology and Engineering support systems and models (27487) models (27236) Taxa - Archaea, Cyanobacteria and Bacteria Taxa - Archaea, Cyanobacteria and Bacteria Eubacteria (19572) Proteobacteria (11924) gamma-Proteobacteria (8569) Enterobacteriales (5170) Enterobacteriaceae (5167) Escherichia (3176) Escherichia coli (3139) Abstract of Article: Will be presented in next release Date Last Accessed: Accessed January 20, 2015 Title of Article: Combination of site-directed mutagenesis and calcium ion addition for enhanced production of thermostable MBP-fused heparinase I in recombinant Escherichia coli Author(s) of Article: Will be identified in next release Citation: Applied microbiology and biotechnology 2013 v.97 no.7 pp. 2907-2916 Click to View: (Click to View) Date of Publication: 2013 Location of Article: http://pubag.nal.usda.gov/pubag/article.xhtml?id=439096 Name of Publication: Applied microbiology and biotechnology Publishing Company: Will be identified in next release Accession Number: 75190