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Biological Sciences: genetics > molecular genetics > genetic engineering > protein engineering
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Breeding and Genetic Improvement: genetic resources
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Items 1 to 40 of 84 results
Group by: Biological Sciences, Breeding and Genetic Improvement
 
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81
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Identifying Functionally Important Mutations from Phenotypically Diverse Sequence Data
Applied and environmental microbiology AEM

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Molecular Determinants of the Cofactor Specificity of Ribitol Dehydrogenase, a Short-Chain Dehydrogenase/Reductase
Applied and environmental microbiology AEM.

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Alteration of Leucine Aminopeptidase from Streptomyces septatus TH-2 to Phenylalanine Aminopeptidase by Site-Directed Mutagenesis
Applied and environmental microbiology AEM

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Proteomic understanding of intracellular responses of recombinant Chinese hamster ovary cells cultivated in serum-free medium supplemented with hydrolysates
Applied microbiology and biotechnology.

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Isolation and functional characterization of an influx silicon transporter in two pumpkin cultivars contrasting in silicon accumulation
The plant journal

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Arginine Residues in the C-terminal and their Relationship with the Analgesic Activity of the Toxin from the Chinese Scorpion Buthus martensii Karsch (BmK AGP-SYPU1)
Applied biochemistry and biotechnology

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Xylanase XYL1p from Scytalidium acidophilum: Site-directed mutagenesis and acidophilic adaptation
Bioresource technology

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Fumarate and cytosolic pH as modulators of the synthesis or consumption of C₄ organic acids through NADP-malic enzyme in Arabidopsis thaliana
Plant molecular biology

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Compensation of the enantioselectivity-activity trade-off in the directed evolution of an esterase from Rhodobacter sphaeroides by site-directed saturation mutagenesis
Applied microbiology and biotechnology

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Analysis of a Ferric Uptake Regulator (Fur) Mutant of Desulfovibrio vulgaris Hildenborough
Applied and environmental microbiology AEM

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Biocatalytic Conversion of Avermectin to 4''-Oxo-Avermectin: Improvement of Cytochrome P450 Monooxygenase Specificity by Directed Evolution
Applied and environmental microbiology AEM

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Introduction of sulfhydryl groups and disulfide linkage to mungbean 8Sα globulin and effects on physicochemical and functional properties
Food research international

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Biochemical and genetic analysis of a cutinase-type polyesterase from a thermophilic Thermobifida alba AHK119
Applied microbiology and biotechnology.

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Crystal structure of Clostridium thermocellum ribose-5-phosphate isomerase B reveals properties critical for fast enzyme kinetics
Applied microbiology and biotechnology.

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How is the reactivity of laccase affected by single-point mutations? Engineering laccase for improved activity towards sterically demanding substrates
Applied microbiology and biotechnology.

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Site-directed mutagenesis of the Arabidopsis heterotrimeric G protein β subunit suggests divergent mechanisms of effector activation between plant and animal G proteins
Planta

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Simple and efficient expression of Agaricus meleagris pyranose dehydrogenase in Pichia pastoris
Applied microbiology and biotechnology.

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New strategy of site-directed mutagenesis identifies new sites to improve Streptomyces clavuligerus deacetoxycephalosporin C synthase activity toward penicillin G
Applied microbiology and biotechnology

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Cloning and sequencing of the α-amylase gene from bacillus subtilis us116 strain encoding an enzyme closely identical to that from bacillus amyloliquefaciens but distinct in thermal stability
Journal of food biochemistry

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Characterization of the necrosis determinant of the European genotype of pepino mosaic virus by site-specific mutagenesis of an infectious cDNA clone
Archives of virology.

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Approaches to achieve high-level heterologous protein production in plants
Plant biotechnology journal

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Transformation of the Green Alga Haematococcus pluvialis with a Phytoene Desaturase for Accelerated Astaxanthin Biosynthesis
Applied and environmental microbiology AEM

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Genetic Modification of the Penicillin G Acylase Surface To Improve Its Reversible Immobilization on Ionic Exchangers
Applied and environmental microbiology AEM

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Identification of activity related amino acid mutations of a GH9 termite cellulase
Bioresource technology

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A single amino acid substitution in PthA of Xanthomonas axonopodis pv. citri altering canker formation on grapefruit leaves
European journal of plant pathology

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Active site analysis of cis-epoxysuccinate hydrolase from Nocardia tartaricans using homology modeling and site-directed mutagenesis
Applied microbiology and biotechnology

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Mitsuaria chitosanase with unrevealed important amino acid residues: characterization and enhanced production in Pichia pastoris
Applied microbiology and biotechnology

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A high-throughput screening system for the evaluation of biomass-hydrolyzing glycoside hydrolases
Bioresource technology

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Three amino acid changes contribute markedly to the thermostability of β-glucosidase BglC from Thermobifida fusca
Bioresource technology

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Enhancement of the thermostability of the maltogenic amylase MAUS149 by Gly312Ala and Lys436Arg substitutions
Bioresource technology

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Analysis of Amino Acid Residues Involved in Catalysis of Polyethylene Glycol Dehydrogenase from Sphingopyxis terrae, Using Three-Dimensional Molecular Modeling-Based Kinetic Characterization of Mutants
Applied and environmental microbiology AEM

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Multiple mutations of the critical amino acid residues for the sweetness of the sweet-tasting protein, brazzein
Food chemistry

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Altering the scissile fatty acid binding site of Candida antarctica lipase A by protein engineering for the selective hydrolysis of medium chain fatty acids
European journal of lipid science and technology

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Inhibition of Cryptosporidium parvum infection of a mammalian cell culture by recombinant scFv antibodies
Antonie van Leeuwenhoek

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A targeted gene knockout method using a newly constructed temperature-sensitive plasmid mediated homologous recombination in Bifidobacterium longum
Applied microbiology and biotechnology.

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The targeting of the oxysterol-binding protein ORP3a to the endoplasmic reticulum relies on the plant VAP33 homolog PVA12
The plant journal

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Functional analysis of NLP genes from Botrytis elliptica
Molecular plant pathology

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Lysine Decarboxylase Catalyzes the First Step of Quinolizidine Alkaloid Biosynthesis and Coevolved with Alkaloid Production in Leguminosae
The plant cell

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Identification and Mutational Analysis of Arabidopsis FLS2 Leucine-Rich Repeat Domain Residues That Contribute to Flagellin Perception
The plant cell

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Engineering of LadA for enhanced hexadecane oxidation using random- and site-directed mutagenesis
Applied microbiology and biotechnology.

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